Shameik Moore Miles Morales / Spider-Man (voice) Hailee Steinfeld Gwen Stacy / Spider-Woman (voice) Brian Tyree Henry Jeff Morales (voice) Luna Lauren Velez Rio Morales (voice) Jake Johnson Peter B. Parker / Spider-Man (voice) Oscar Isaac Miguel O'Hara / Spider-Man 2099 (voice) Jason Schwartzman Jonathan Ohnn / Spot (voice) Issa Rae Jessica Drew / Spider-Woman (voice) Daniel Kaluuya Hobie Brown / Spider-Punk (voice) Karan Soni Pavitr Prabhakar / Spider-Man India (voice) Shea Whigham George Stacy (voice) Greta Lee LYLA (voice) Mahershala Ali Uncle Aaron (voice) Amandla Stenberg Margo Kess / Spider-Byte (voice) Jharrel Jerome Miles G. Morales (voice) Andy Samberg Ben Reilly / Scarlet Spider (voice) Jack Quaid Peter Parker / The Lizard (Voice) Rachel Dratch Ms. Weber (voice) Ziggy Marley Lenny (voice) Jorma Taccone Adriano Tumino / Medieval Vulture / '67 Spider-Man (voice) J.K. Simmons J. Jonah Jameson (voice) Ayo Edebiri Glory (voice) Sofia Barclay Malala Windsor / Spider-UK (voice) Alfred Molina Doctor Octopus (voice) (archive footage) (uncredited) Post Malone Bystander (voice) (archive footage) (uncredited)