Shameik Moore Miles Morales / Spider-Man (voice) Hailee Steinfeld Gwen Stacy / Spider-Woman (voice) Oscar Isaac Miguel O'Hara / Spider-Man 2099 (voice) Jake Johnson Peter B. Parker / Spider-Man (voice) Issa Rae Jessica Drew / Spider-Woman (voice) Daniel Kaluuya Hobart 'Hobie' Brown / Spider-Punk (voice) Jason Schwartzman Jonathan Ohnn / Spot (voice) Nicolas Cage Peter Parker / Spider-Man Noir (voice) John Mulaney Peter Porker / Spider-Ham (voice) Kimiko Glenn Peni Parker (voice) Amandla Stenberg Margo Kess / Spider-Byte (voice) Karan Soni Pavitr Prabhakar / Spider-Man (voice) Brian Tyree Henry Jefferson Davis (voice) Luna Lauren Velez Rio Morales (voice) Mahershala Ali Uncle Aaron (voice) Jharrel Jerome Miles G. Morales / Prowler (voice)